Nitrogen Cycle (Edexcel IGCSE Biology)
The Nitrogen Cycle
Spec point —
5.11B· describe the stages in the nitrogen cycle, including the roles of nitrogen fixing bacteria, decomposers, nitrifying bacteria and denitrifying bacteria (specific names of bacteria are not required)
- Nitrogen is present as N2gas in the atmosphere and within biological molecules, such as proteins, in the tissues of living organisms
- Nitrogen is cycled through ecosystems via the nitrogen cycle
Nitrogen fixation
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Nitrogen fixation makes nitrogen available to living organisms
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Nitrogen fixing bacteria convert N2gas into ammonium compounds, which are then converted into nitrates in the soil
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Nitrogen fixing bacteria include:
- Free-living bacteria in the soil
- Bacteria in the root nodules of leguminous plants (e.g. peas, beans, clover) which form a mutualistic relationship with the plant
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Nitrogen can also be fixed by lightning or during the production of chemical fertilisers
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After nitrogen fixation has occurred plants absorb nitrates from the soil to build plant proteins
Transfer of nitrogen between living organisms
- Animals feed on plants, digest the proteins in the plant tissues, providing nitrogen to build animal proteins
- Nitrogen may then be passed from one consumer to another up the food chain in the same way
Release of nitrogen from tissues
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Nitrogen from dead organisms and metabolic waste products is returned to the soil as ammonia by decomposers (bacteria and fungi)
- Ammonia reacts with water in the soil to form ammonium ions
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Plants can’t absorb ammonium ions directly, so nitrifying bacteria convert ammonia into nitrites, and then into nitrates, which can be taken up again by plants
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The conversion of ammonium compounds to nitrates is known as nitrification, and can be summarised as follows:
ammonia → nitrites → nitrates
Denitrification
- Denitrifying bacteria convert nitrates back into N₂ gas, returning nitrogen to the atmosphere. This process is known as denitrification
- Denitrifying bacteria are active in anaerobic conditions, e.g. in waterlogged or compacted soil
- Farmers reduce denitrification by ploughing the soil to increase aeration

The nitrogen cycle involves nitrogen fixation, decomposition, nitrification and denitrification
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