Investigating the Differences in Inspired & Expired Air (Cambridge International IGCSE Biology)
Exam board Cambridge International · IGCSEUpdated 2026-08-28
Spec point —
11.1.3· Investigate the differences in composition between inspired and expired air using limewater as a test for carbon dioxide
Investigating the differences in inspired & expired air
- A simple experimental setup can be used to investigate the differences between inspired and expired air

The limewater test
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When air is breathed in, it is drawn through boiling tube A
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When air is breathed out, the air is blown into boiling tube B
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Limewater is clear but becomes cloudy (or milky) when carbon dioxide is bubbled through it
- The limewater in boiling tube A will remain clear, as inhaled air is atmospheric air, which contains a very low percentage of carbon dioxide The limewater in boiling tube B will become cloudy, as exhaled air has passed through the lungs (where gas exchange has taken place) and contains a higher percentage of carbon dioxide
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This shows us that the percentage of carbon dioxide in exhaled air is higher than in inhaled air
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